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Image Search Results
Journal: Nature Communications
Article Title: Efficient active hydrogen delivery for drug-free radiation enteritis therapy in mice
doi: 10.1038/s41467-025-64270-9
Figure Lengend Snippet: a Schematic illustration of the radioprotective mechanism of H x MoO 3 @SA@COS nanomachines. b Cell viability of HIEC-6, MODEK and RAW264.7 cells treated with different concentrations of H x MoO 3 @SA@COSs. c Radioprotective effects of H x MoO 3 @SA@COSs on HIEC-6 cells at various radiation doses, with significance compared to controls. d − i Representative fluorescence images showing the uptake profile of H x MoO 3 @SA@COSs ( d ) and intracellular ROS levels ( e ) in HIEC-6 cells under different treatments, flow cytometry analysis ( f ) using Annexin V-FITC/PI staining was performed to assess apoptosis and necrosis, with corresponding quantitative results for mean fluorescence intensity (MFI) of FITC ( g ) and DCFH-DA ( h ), along with cell apoptosis percentages ( i ). Experiments were independently repeated three times ( d ) or five times ( e ) with similar results. j , l Immunofluorescence images of γ-H2AX showing DNA double-strand breaks ( j ), with corresponding quantification ( l ). k − n Macrophage polarization, visualized via CD86 (M1) and CD206 (M2) expression ( k ), with quantitative results ( m, n ). n = 3 ( b − n ) or n = 5 ( h , l ) biologically independent replicates, data are presented as mean ± SD (one way ANOVA and Tukey′s multiple comparisons tests). Source data are provided as a Source Data file.
Article Snippet: Total Antioxidant Capacity Assay Kit with ABTS method, Total Superoxide Dismutase Assay Kit with WST-8, Lipid Peroxidation MDA Assay Kit, Reactive Oxygen Species Assay Kit, Annexin V-FITC Apoptosis Detection Kit,
Techniques: Fluorescence, Flow Cytometry, Staining, Immunofluorescence, Expressing